绵羊虱蝇形态学与分子生物学鉴定

doi:10.11843/j.issn.0366-6964.2017.11.020

Abstract

Abstract:

Melophagus ovinus is one of the most common and economically important blood-feeding ectoparasites on sheep, but no exhaustive information on the morphology of M. ovinus. The aim of this study was to provide the basis for rapid and accurate identify this parasite. The morphological characteristics of M. ovinus were observed by the super depth of field three-dimensional microscope system and scanning electron microscope, and the molecular identification was also performed by analyzing the gene sequence of 18S rRNA. The results showed that M. ovinus was an entirely wingless brown fly, no halteres. The head was short and broad flat. M. ovinus had piercing-sucking mouthparts, reduced compound eyes and thumbnail antennae, no ocellus. The chest was not segmented, had three pairs of legs and four pairs of stegmas, and the tibial ends have large claws. The abdomen was wide and ameristic, with an oval or round shape. The abdomens of M. ovinus differ depending on the sex:the female's abdomen was big, round, and invaginated in the back; the male's abdomen was small, round, and embossed in the back. The entire surface of the body had dense setae. The sequencing result of 18S rRNA had 99.59% similarity with the published sequences in GenBank. These findings suggested that the samples obtained from the sheep bred in the city of Jiuquan in Gansu Province were M. ovinus, and this parasite had easily recognizable morphological characteristics.

CLC Number:

S852.74

DUAN De-yong, WANG Ya-qin, CHENG Tian-yin. Morphological and Molecular Identification of Melophagus ovinus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2181-2188.

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References

[1] GIBSON W, PILKINGTON J G, PEMBERTON J M. Trypanosoma melophagium from the sheep ked Melophagus ovinus on the island of St Kilda[J]. Parasitology, 2010, 137(12):1799-1804.
[2] SMALL R W. A review of Melophagus ovinus (L.), the sheep ked[J]. Vet Parasitol, 2005, 130(1-2):141-155.
[3] KUMSA B, PAROLA P, RAOULT D, et al. Bartonella melophagi in Melophagus ovinus (sheep ked) collected from sheep in northern Oromia, Ethiopia[J]. Comp Immunol Microbiol Infect Dis, 2014, 37(1):69-76.
[4] KETTLE D S. Medical and veterinary entomology[M]. London:Croom Helm, 1984:658.
[5] 王勇,孙宝杰,于长友, 等. 临沂口岸截获加拿大入境蝇类:羊虱蝇[J]. 中国媒介生物学及控制杂志, 2010, 21(1):35.
WANG Y, SUN B J, YU C Y, et al. Melophagus ovinus Linnaeus intercepted and captured from Canadian pickled goatskins at Linyi port[J]. Chinese Journal of Vector Biology and Control, 2010, 21(1):35. (in Chinese)
[6] LIU D, WANG Y Z, ZHANG H, et al. First report of Rickettsia raoultii and R. slovaca in Melophagus ovinus, the sheep ked[J]. Parasit Vectors, 2016, 9:600.
[7] BEQUAERT J. A monograph of the Melophaginae, or ked-flies, of sheep, goats, deer and antelopes (Diptera, Hippoboscidae)[J].Entomol Am, 1942, 22:1-220.
[8] TETLEY J H. The sheep ked, Melophagus ovinus L. I. Dissemination potential[J]. Parasitology, 1958, 48(3-4):353-363.
[9] LASSNIG H, PROSL H, HINTERDORFER F. Parasites of the red fox (Vulpes vulpes) in Styria[J]. Wien Tierarztl Monatsschr, 1998, 85(4):116-122.
[10] LUEDKE A J, JOCHIM M M, BOWNE J G. Preliminary bluetongue transmission with the sheep ked Melophagus ovinus (L.)[J]. Can J Comp Med Vet Sci, 1965, 29(9):229-231.
[11] CHU C Y, JIANG B G, QIU E C, et al. Borrelia burgdorferi sensu lato in sheep keds (Melophagus ovinus), Tibet, China[J]. Vet Microbiol, 2011, 149(3-4):526-529.
[12] HORNOK S, DE LA FUENTE J, BIRÓ N, et al. First molecular evidence of Anaplasma ovis and Rickettsia spp. in keds (Diptera:Hippoboscidae) of sheep and wild ruminants[J]. Vector Borne Zoonotic Dis, 2011, 11(10):1319-1321.
[13] 姜昌武, 张守发, 金钟羲. 绵羊的羊蜱蝇病诊疗观察[J]. 延边农学院学报, 1983, 5(2):62-64.
JIANG C W, ZHANG S F, JIN Z X. Treatment and observation the disease of Melophagus ovinus on sheep[J]. Journal of Agricultural Science Yanbian University, 1983, 5(2):62-64. (in Chinese)
[14] 薛晶, 金映红, 王光雷, 等. 新疆青河县羊蜱(虱)蝇病的诊治报告[J]. 中国兽医杂志, 2016, 52(6):51-52.
XUE J, JIN Y H, WANG G L, et al. Report on diagnosis and treatment of the disease of Melophagus ovinus in Qinggil County of Xinjiang[J]. Chinese Journal of Veterinary Medicine, 2016, 52(6):51-52. (in Chinese)
[15] ZHANG D, LIU X H, LI X Y, et al. Ultrastructural investigation of antennae in three cutaneous myiasis flies:Melophagus ovinus, Hippobosca equina, and Hippobosca longipennis (Diptera:Hippoboscidae)[J]. Parasitol Res, 2015, 114(5):1887-1896.
[16] WATERS A P, MCCUTHAN T F. Ribosomal RNA:Nature's own polymerase-amplified target for diagnosis[J]. Parasitol Today, 1990, 6(2):56-59.
[17] COSSIO-BAYUGAR R, PILLARS R, SCHLATER J, et al. Theileria buffeli infection of a Michigan cow confirmed by small subunit ribosomal RNA gene analysis[J]. J Vet Parasitol, 2002, 105(2):105-110.
[18] 汪晓阳, 周志军, 黄原, 等. 基于18S rRNA基因序列的直翅目主要类群系统发育关系研究[J]. 动物分类学报, 2011, 36(3):627-638.
WANG X Y, ZHOU Z J, HUANG Y, et al. The phylogenetic relationships of higher orthopteran categories inferred from 18S rRNA gene sequences[J]. Acta Zootaxonomica Sinica, 2011, 36(3):627-638. (in Chinese)
[19] MUNGUIRA M L, MARTíN J, GARCÍA-BARROS E, et al. Morphology and morphometry of Lycaenid eggs (Lepidoptera:Lycaenidae)[J]. Zootaxa, 2015, 3937(2):201-247.
[20] JIANG L, GAO Q H, HUA B Z. Larval morphology of the hanging-fly Bittacus trapezoideus Huang & Hua (Insecta:Mecoptera:Bittacidae)[J]. Zootaxa, 2015, 3957(3):324-333.
[21] ÁLVAREZ M, MUNGUIRA M L, MARTÍNEZ-IBÁÑEZ M D. Comparative study of the morphology of stridulatory organs of the Iberian Lycaenid butterfly pupae (Lepidoptera)[J]. J Morphol, 2014, 275(4):414-430.
[22] ARIF Y, LOMBARKIA N. Morphological characteristics of the sensilla ovipositor, tarsus and antenna in dates moth female, Ectomyelois ceratoniae (Lepidoptera:Pyralidae)[J]. Acad J Entomol, 2015, 8(2):34-37.
[23] LEHNERT M S, MONAENKOVA D, ANDRUKH T, et al. Hydrophobic-hydrophilic dichotomy of the butterfly proboscis[J]. J R Soc Interface, 2013, 10(85):20130336.
[24] SEADA M A. Antennal morphology and sensillum distribution of female cotton leaf worm Spodoptera littoralis (Lepidoptera:Noctuidae)[J]. J Basic Appl Zool, 2015, 68:10-18.
[25] 黄兵, 沈杰. 中国畜禽寄生虫形态分类图谱[M]. 北京:中国农业科学技术出版社, 2006.
HUANG B, SHEN J. Classific atlas of parasites for livestock and poultry in China[M]. Beijing:China Agricultural Science and Technology Press, 2006. (in Chinese)
[26] SOKÓ? R, MICHALSKI M M. Occurrence of Hippobosca equina in Polish primitive horses during the grazing season[J]. Ann Parasitol, 2015, 61(2):119-124.
[27] 刘进辉, 彭俊宇, 宋海燕, 等. 10株火鸡组织滴虫18S rRNA基因的克隆及进化分析[J]. 畜牧兽医学报, 2011, 42(3):448-452.
LIU J H, PENG J Y, SONG H Y, et al. Cloning and phylogenetic analysis of the 18S rRNA of Histomonas meleagridis isolates from Hunan province[J]. Acta Veterinaria et Zootechnica Sinica, 2011, 42(3):448-452. (in Chinese)

Morphological and Molecular Identification of Melophagus ovinus

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